Sperm cells used in IVF techniques require the adequate preparation. With the use of techniques of sperm cell isolation from semen, spermatozoa are separated from the semen plasma, substances inhibiting the ability to fertilise egg cells are removed, processes facilitating the egg cell fertilisation are initiated, sperm cells with proper morphology and motility are selected, bacteria and viruses are eliminated and the sperm cells with a female or male chromosome are selected.
The following techniques are most commonly applied in the sperm cell isolation:
The most commonly used method of sperm separation is density gradient centrifugation (DGC), or the Percoll method, which allows to obtain the homogeneous population of normal sperm cells, separate the sperm cells from other morphotic elements of semen, and poses negligible risk of losing some small amount of spermatozoa when their number in the ejaculate is small.
Do you know that....
INVICTA Fertility Clinic was the first in Poland to perform, in 2008, Sperm Separation Test in its own Medical Laboratories!